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KMID : 0603820080140030167
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Journal of Experimental & Biomedical Science
2008 Volume.14 No. 3 p.167 ~ p.172
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Histologic Evaluation of Collagen Generation in Mouse Subcutaneous Tissue Using 880 ㎚ & 630 ㎚ LED
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Ahn Jin-Chul
Hwang Hee-Jun
Chung Phil-Sang
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Abstract
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We compared the clinical efficacy of LED therapy using 880 ㎚ and 630 ㎚ LED to test collagen accumulations in subcutaneous tissue of mouse after LED irradiation by measuring the quantity of collagen. 880 ㎚ and 630 ㎚ LED was irradiated on the back of ICR mouse given at 10.8 J/cm2 followed for 30 minutes everyday for 5 weeks. Histological observation was performed by Hematoxylin & Eosin staining and Masson’s Trichrome collagen staining. We also used Sircol soluble collagen assay kit for measuring the amounts of collagen in the mouse skin tissue after 1, 3, and 5 weeks post LED irradiation, respectively. Collagen generation was found at subcutaneous tissue, and the quantity of collagen in 880 ㎚ LED group had grown more than that of 630 ㎚ LED group at 5 weeks follow-up later. About 75% more efficacies for collagen generation were found in the group of 5th week of 880 ㎚ LED irradiation. The efficacy of 880 ㎚ LED could be more useful than 630 ㎚ LED for synthesizing collagens in mouse subcutaneous tissue as time followed.
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KEYWORD
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Phototherapy, LED (880 ㎚ &, 630 ㎚), Collagen
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